Lycoperdon pyriforme used in is traditional medicine by locals in different parts of Ethiopia. The study aimed to evaluate the antioxidant, antimicrobial and phytochemical constituents of the methanol extract of Lycoperdon pyriforme which may infer the antioxidant and antimicrobial activity of this fungi. DPPH radical scavenging, total phenol content assay and total flavonoid content assay were done to determine the antioxidant activity of the fungi extract. Agar disc diffusion method was used to determine the antimicrobial properties against S. aureus, B. subtilis, C. albicans, E. coli in a dose-dependent manner. The fungi extract was found to show significant antimicrobial and antioxidant properties. The presence of phenol and flavonoid compounds present in the fungi could be the reason behind the antioxidant and antimicrobial activity. The current study shows that Lycoperdon pyriforme could be a good source for preparation of antibacterial, antifungal and antioxidant extracts in future.
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