Agricultural and Biological Research

Phenotypic characterization and CBNAAT diagnosis of MTB from pulmonary and extra pulmonary samples

Harshitha J Naik and G Bhuvaneshwari*

The majority of instances of Tuberculosis (TB) occur in underdeveloped nations, and it is one of the leading causes of morbidity and mortality worldwide. In underdeveloped nations, it is still unclear how important pulmonary and extrapulmonary tuberculosis are compared to other types of the disease. The disease Tuberculosis (TB) is very contagious. Pulmonary tuberculosis is the name given to a disease that typically affects the upper respiratory system of the body. The most typical form of the disease is pulmonary TB. Mycobacterium tuberculosis, a slow-growing Acid-Fast Bacillus (AFB). The respiratory system is the route through which the Mycobacterium is spread. Extra-Pulmonary TB (EPTB) is the name given to a condition that can also lead to tuberculosis in an organ of the body. Identification and phenotypic characterization of clinically significant mycobacterium isolates from pulmonary and extra-pulmonary was performed along CBNAAT diagnosis. A total of 317 Pulmonary and Extrapulmonary specimens were received at IRL, Bangalore were used for this study. Smears were prepared for all pulmonary samples and smears were subjected to acid fast bacillus staining technique. Smear microscopy testing revealed 206 samples to be positive. Liquid culture media MGIT-960 was used to inoculate the 206 Mycobacterium samples that had positive smear results. Additionally, the Cartridge Based Nucleic Acid Amplification Test (CB-NAAT) revealed MTB in 91 Rif resistant, 170 Rif sensitive, 48 samples where MTB was undetectable and 5 samples where Rif intermediate was visible.