Agricultural and Biological Research

Potato bacterial ring rot pathogen detection and outbreak prevention

Mini Chakma*, Meenakshi Rana, Akash Jangid and Anshuman Shringi

Clavibacter michiganensis subsp. sepedonicus (Cms), causing bacterial ring rot (BRR) is one of the most destructive diseases of Potato. It is a soil-borne, gram-positive, non-motile, non-spore-bearing bacterium. It is observed that Cms can grow well in the presence of oxygen as compared to the absence of oxygen therefore it is an aerobic bacterium. Some of the challenges faced due to bacterial ring rot are continuous rejection of potatoes seed/tuber which is continuously affecting potato export markets. Potato tuber is found to be the primary source of inoculum. Latency nature of the disease makes it more difficult to detect at early stage of infection. Even recent diagnostic techniques have limited sensitivity for the detection of Cms in samples of potato seed giving false positive result that can lead to waste of management strategies, time and money. The disease can be diagnosed by using serological methods gram stain, which will denote the presence of Cms pathogen. The pathogen of BRR can be detected with the support of ELISA, PCR, LAMP, and many other techniques that remain undetected due to its latency nature, out of which LAMP is found to be more effective and affordable as it does not require laboratory complex devices and it is comparatively simple, fast, easy to use that can prevent further infection by early-stage detection of the BRR pathogen. PCR methods are more sensitive than other serological methods. From PCR MP (Melting profiles) method, electrophoretic patterns could be identified as well as can distinguish each of the 50 isolates from the other isolates by analyzing with the applied restriction enzyme. Cms can be managed by using disease-free seed or certified seeds, removing debris, host plants, and the use of disinfectants like ammonia, iodine, and chlorine- containing compounds.

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